Histology of your pets in Kyiv
This is a study of a tiny volume of tissue of internal organs. First, the material is taken – particles of the organ. Most often, a histological examination of the material is carried out to confirm the diagnosis of oncology in a patient, and a part of the tumor is examined for its malignancy. In the case of a diagnosis of a cancerous tumor, a histological examination is mandatory, as the most accurate method of diagnosis, and as a method that gives results in the early stages of the disease.
The technique of histological examination consists of several stages:
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- Material collection
- Sample fixation. This is done so that the organ tissue does not “die”. These can be various solutions or acids consisting of one or more components. “Bathing” a tissue particle can take several hours, or maybe several days. It all depends on both the fixative and the material sample.
- The tissue sample is washed in water, then dehydrated. This is done for subsequent filling with paraffin, because it does not dissolve in water.
- The material taken is filled with liquid paraffin, then a series of procedures are performed to completely solidify it. Next, the tissue surrounded by paraffin is formed into blocks.
- Ultrathin sections are made with a special knife – a microtome, the blocks are cut into plates.
- Paraffin is removed from the plates, and they are stained for clear cell outlines.
- They are washed and dehydrated again. And after a long preliminary preparation, they are placed on a microscope slide, lubricated with balsam. The structure of the tissue is studied, and the doctor draws a conclusion.
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